How to use:
Please type in the oligo sequence using the characters below.
A, T, C, G or a, t, c, g
+A +C +T +G or +a +c +t +g
This tool is based on a modified nearest-neighbor themodynamic model and experimental data.
© 2014 Bio-Synthesis, Inc. R&D Division
Information and limitations of the BNA Tm prediction calculator:
The most accurate Tm value can be obtained in the range from 9 to 20 nucleotides.
It is, however, possible to obtain reasonably accurate Tm values in other ranges.
The predicted Tm values are based on the following: